Browsing Genetics by Issue Date
Now showing items 1-20 of 146
-
Analysis of resistance to inhibitors of Plasmodium falciparum dihydrofolate reductase in yeast
(1996)Resistance to the malaria drugs is an increasing problem for treating the 200-300 million people infected yearly with falciparum malaria. Detailed analysis of the development of drug resistance has been complicated by ... -
Genetic, molecular, and cytological characterization of the paternal effect gene, paternal loss, of Drosophila melanogaster
(1996)Mutations in the paternal effect gene, paternal loss (pal) of Drosophila melanogaster cause loss of only the paternally inherited chromosomes during early embryogenesis. In an effort to better understand the role of the ... -
Study of the Cryptosporidium parvum DHFR-TS in the model system Saccharomyces cerevisiae
(1998)Cryptosporidium parvum is a ubiquitous protozoan that causes severe gastrointestinal disease. The severe diarrhea that results can be life-threatening, particularly for the very young, old, and immune-compromised. Currently ... -
Analysis of daf-11, a transmembrane guanylyl cyclase that mediates chemosensory transduction in C. elegans
(1998)C. elegans daf-11 mutants have defects in chemosensory responses to a pheromone and to non-volatile and some volatile attractants. daf-11 encodes a transmembrane guanylyl cyclase (TM-GC) and provides the first example of ... -
Analysis of BRCA1 genomic structure: novel germline mutations and somatic alterations in breast cancer
(2000)Germline mutations in BRCA1 lead to an increased risk of breast and ovarian cancer, with loss of the second, normal allele critical to tumorigenesis. The relative lack of somatic mutations in BRCA1 , however, has argued ... -
MSY4, a sequence-specific RNA binding protein expressed during mouse spermatogenesis
(2000)Translational control is an important mechanism of gene regulation, especially in gametogenesis and early embryogenesis, where there are periods of time in which there is no transcription. Previously transcribed mRNAs must ... -
Insights into mechanisms of Pseudomonas aeruginosa virulence: cyanide as a weapon and the complexity of its regulation
(2001)This dissertation presents investigations into virulence mechanisms and virulence-gene regulatory circuitry in the bacterium Pseudomonas aeruginosa . P. aeruginosa is a medically important human pathogen that causes serious ... -
Genetic analysis of rhythmic behavior in C. elegans
(2005)How do genetic networks control the behavior of an organism? To approach this problem, I chose the model organism C. elegans, a simple metazoan that displays several easily observed behavioral programs such as locomotion, ... -
Genetic and biochemical analysis of the Drosophila melanogaster homolog of the human SCA2 gene
(2005)The polyglutamine repeat diseases are a group of dominantly inherited neurodegenerative disorders characterized by progressive degeneration of specific neuronal populations and a shared mutational mechanism involving ... -
Establishment of a Drosophila model of intestinal sterol absorption and trafficking
(2007)Despite diet being a major source of cholesterol in Western culture, the absorption and downstream trafficking of cholesterol remain poorly understood processes. In humans, proteins of the NPC1 family are essential for ... -
Genetic profiling of drug resistance in Plasmodium falciparum
(2007)Malaria is an infectious febrile illness caused by four species of Plasmodium parasite, with Plasmodium falciparum responsible for the greatest number of fatalities. Unfortunately, drug resistant strains of P. falciparum ... -
A practically efficient graph-theoretic approach to protein identification in mass spectrometry
(University of Washington Graduate School, 2011-06)Tandem mass spectrometry has emerged as a powerful tool for the characterization of complex protein samples, an increasingly important problem in biology. The effort to efficiently and accurately perform inference on ... -
Using exome sequencing to study adaptive evolution in non-human primates and human populations
(University of Washington Graduate School, 2012)Positive selection promotes the fixation of functional genetic differences. Studying these adaptive differences can provide insights into how species adapt to their environment and develop their unique phenotypes. In this ... -
Tools and Analyses for Differential Label-Free Proteomics Using Mass Spectrometry
(2012-08-10)The comparative measurement of protein abundance is a powerful method to detect changes in the biological dynamics of cells and tissues. Shotgun proteomics has proven to be a method where a wide range of proteins can be ... -
Development of an Allele-Aware Method to Study the Nuclear Organization of the FSHD Locus
(2012-09-13)The human genome carries out its functions, including the establishment and maintenance of tissue-specific gene expression programs, within the confines of the cell nucleus. The genome is not randomly organized within this ... -
Androgen Receptor Regulation of Germ Cell Migration across Sertoli Cell Tight Junctions
(2012-09-13)The blood-testis barrier includes a tight junction barrier between Sertoli cells that restricts solutes of varying size and charge from crossing the paracellular space thus enabling the formation of unique microenvironments ... -
Next Generation Mendelian Genetics
(2013-02-25)The study of Mendelian disorders has been of immense utility in uncovering the genetic and molecular basis of numerous human traits, and has greatly furthered the identification of genes, the annotation of gene function ... -
Massively parallel functional dissection of regulatory elements
(2013-02-25)Massively parallel sequencing has accelerated the cataloging of cis-regulatory elements in mammalian genomes. However, it remains challenging to estimate the functional effects of variation in cis-regulatory elements. The ... -
Molecular tagging to overcome limitations of massively parallel sequencing
(2013-04-17)Massively parallel technologies have dramatically reduced the cost and increased the throughput of DNA sequencing, transforming the study of patterns of genetic variation in human and model organisms, the genetic basis of ... -
Conflict and Coevolution Shape the Primate Kinetochore
(2013-04-17)Centromeres and the kinetochore proteins that bind them are required for chromosome segregation during every eukaryotic cell division. Despite this conserved function, ongoing conflict between selfish centromeric DNA and ...