Browsing Molecular and cellular biology by Title
Now showing items 13-32 of 142
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B cells promote inflammatory T cell responses during the initiation of central nervous system autoimmune disease
(2013-07-25)Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS) mediated by autoreactive T cells specific for myelin proteins. A recent development in MS treatment is the discovery that ... -
A beneficial toxin: The response to hydrogen sulfide improves survival in a changing environment
Fluctuations in environmental conditions can be deadly. The ability to rapidly and appropriately respond to stressful conditions can mean the difference between life and death. The toxic gas hydrogen sulfide (H2S) is a ... -
BRCA genes: conserved regions and the potential effect of missense changes
(2005)Half of coding single-nucleotide polymorphisms in the human genome result in missense changes. Many are rare, weakly penetrant, or occur in genes for which functional assays are not available. Comparative analysis of protein ... -
Bypassing androgen pathway dependence in advanced prostate cancer.
(2012-09-13)Continued reliance of castration-resistant prostate cancer (CRPC) on androgen receptor (AR) signaling has spurred the development of novel, potent antiandrogen therapeutics. The clinical use of successively more potent ... -
C. elegans Germline Defense Against Retrotransposons
(2012-09-13)Nematodes appear to have efficient defenses against the germline activity of retroviruses and retrotransposons, as evidenced by the small number of sequences in their genome derived from such elements. No viruses that ... -
Centromere identification and inactivation on a neo-Y chromosome fusion in threespine stickleback fish (Gasterosteus aculeatus)
Centromeres are the primary constriction observed on many chromosomes, and they are required for normal cell division. Having one and only one centromere per chromosome is essential for proper chromosome segregation during ... -
Characterization of antagonistic interbacterial pathways at the single-cell level
Antagonistic pathways are nearly universal among free-living bacteria, and may determine which organisms co-exist in polymicrobial communities. Bacteria have also evolved pathways that can sense and respond to assaults ... -
Characterization of Mitochondrial DNA Mutations in Drosophila melanogaster
(2014-04-30)Mitochondria are vital organelles as they produce the majority of cellular energy in the form of ATP through oxidative phosphorylation (OXPHOS). Mitochondria contain their own genome (mtDNA), which encodes essential ... -
Characterization of novel, context-dependent modulators of WNT signaling in cancer
(2013-07-23)WNTs comprise a family of secreted proteins that play critical roles in both embryogenesis and in the etiology of human diseases such as cancer. Even after more than three decades of research, our understanding of the ... -
Characterization of organelle morphology and DNA repair in replicatively aging yeast
(2013-11-14)Aging is one of the most ubiquitous processes in biology, and yet, how the aging process is initiated and the mechanisms that govern how an organism ages remain largely unknown. The study of human aging has been impeded ... -
Characterization of the Ipl1/Aurora protein kinase in chromosome segregation and the spindle checkpoint
(2005)The flawless execution of cell division is fundamental to the formation and survival of living organisms. It depends on the accurate and orderly partitioning of chromosomes, resulting in two daughter cells with the correct ... -
Characterization of the Isw1a and Isw1b ATP-dependent chromatin remodeling complexes from the budding yeast, Saccharomyces cerevisiae
(2003)Members of the ISWI class of ATP-dependent chromatin remodeling complexes have been found in nearly every eukaryote studied, suggesting important functions that have been conserved throughout evolution. The budding yeast, ... -
Characterization of the Warburg Effect and Crabtree effect in Barrett's Esophagus Cell lines and Development of a Chip-Based Single-Cell Respirometry System
(2012-05-31)This thesis consisted of two distinct parts: In the first part, Warburg and Crabtree effects on energy metabolism were characterized in cell lines derived from premalignant Barrett's esophagus patients; in the second part, ... -
Characterizing the neutralizing antibody responses of HIV-1 superinfected individuals
Eliciting a neutralizing antibody (Nab) response that is protective against diverse HIV-1 variants presents a major challenge to vaccine development. The identification of broad, cross-reactive Nabs from HIV-infected ... -
Chromatin landscape of the "dark matter of the genome": centromeres of S. cerevisiae and repeat sequences of D. melanogaster.
The chromatin landscape plays a major role in defining cell phenotypes through transcriptional regulation and specification of the main features of chromosome, such as centromeres and pericentric heterochromatin. Studies ... -
Chromatin Remodeling Around Nucleosome Free Regions Represses Non-Coding RNA By DNA Looping and Transcription Factor Dependent Targeting of Isw2
(2013-02-25)The efficient three-dimensional packaging of DNA into eukaryotic nuclei is accomplished through spatially organizing and compacting DNA into chromatin. Maintaining proper access to DNA, by modulation of either the location ... -
Comprehensive, precision genomics
(2014-04-30)The past decade has observed a significant drop in the cost-per-base of DNA sequencing. Driven by a new era of `next-generation' sequencing (NGS), there has been an explosion of new technologies that utilize DNA sequencing, ... -
Computational Design and Directed Evolution of Novel Enzymes
(2013-11-14)Enzymes are the most specific and active catalysts found in nature, and offer unique chemical properties suited to solving important industrial, medical, and environmental problems. In this work I present new methods for ... -
Computational design of co-assembling multi-component protein nanomaterials
Molecular self- and co-assembly of proteins into highly ordered symmetric complexes is an elegant and powerful means of patterning matter at the atomic scale and a hallmark of biological systems. Inspired by the exquisite ... -
Computational design of protein-protein interactions to engineer novel inhibitors of the p53 pathway and the polycomb repressive complex
Here I present 2 examples of the application of the Rosetta macromolecular modeling software suite to the successful development of novel protein-based inhibitors with the aim of creating useful tools for the greater cancer ...