Browsing Pathology by Title
Now showing items 1-20 of 60
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A genome-scale gain-of-function screening platform nominates genetic modulators for CAR T cell therapy enhancement
Chimeric antigen receptor (CAR) T cell therapy has revolutionized cancer care through genetic reprogramming, and next-generation cell engineering aims to encode increasingly complex genetic programs to further modulate T ... -
An Automated System For High-Throughput Longevity and Healthspan Discovery in Caenorhabditis elegans
Over the last century, the study of aging biology has primarily been advanced through the development and use of animal models that share common molecular hallmarks with human aging. One major model system that has been ... -
Cell-specific transcriptional analysis in complex tissues
Cell-type specific transcriptional analysis can yield important information about the role of a certain cell type in the context of its tissue microenvironment. Previous methods for isolating cell type-specific transcriptional ... -
A cell-type specific approach to assess the contribution of dysregulated nutrient handling to atherosclerosis
The severity of atherosclerosis, which underlies the majority of cardiovascular disease, is determined by a wide variety of factors. Though many of these factors are well studied, a complete understanding of how to best ... -
The Cellular and Molecular Axis of Muscle Regeneration
Skeletal muscle has significant regenerative capacity, which is impaired with muscular dystrophy and aging. Muscle function and repair requires the involvement of several cellular compartments and molecular interactions. ... -
Characterization of Precancerous Mutations in Ulcerative-Colitis Associated Colorectal Cancer and High-Grade Serous Ovarian Cancer via Duplex Sequencing
Humans have attempted to fight cancer since the beginning of recorded history, but advances in the early detection of cancer have begun to emerge only in the last several decades. Patient survival increases with earlier ... -
Characterizing the role of DDX3X in DNA double strand break repair; Implications for lymphocyte biology
DDX3X is a human, ATP-dependent RNA helicase with roles throughout RNA metabolism. A number of human diseases are associated with alterations in this gene, particularly in hematological malignancies. We aimed to investigate ... -
Comprehensive analysis of WRN protein interaction network by Mass Spectrometry.
(2014-04-30)Cells that have lost WRN function exhibit a shortened replicative lifespan, accumulation of chromosomal aberrations, and demonstrate sensitivity to a number of chemotherapeutic agents, including DNA Topoisomerase I inhibitor ... -
Deciphering the relationship among nutrition, host and microbe
Within the human gastrointestinal tract, there are trillions of resident microbes collectively known as the gut microbiota. These organisms have a profound impact on host physiology, particularly the immune system. Upon ... -
Defective branched chain amino acid catabolism impairs exercise capacity and glucose homeostasis in the mouse
The branched-chain amino acids (BCAAs) leucine, isoleucine and valine, play a central role in substrate metabolism, energetics, and protein synthesis. Although BCAA supplementation is widely used to improve exercise capacity ... -
Development and characterization of a new assay to examine telomere-protein interactions in vivo
(1997)An in vivo telomere binding assay using one-hybrid methodology was devised. A reporter gene, lacZ, was integrated immediately adjacent to a chromosomal telomere in Saccharomyces cerevisiae. If a telomere binding protein ... -
Development of Enzyme Replacement Therapy in Mammalian Models of Barth Syndrome
Barth Syndrome (BTHS) is a devastating disorder caused by a single gene mutation in the mitochondrial transacylase, tafazzin (TAZ), which results in impaired lipid metabolism leading to dysfunction in highly energetic ... -
Enhancing Local delivery of Macrophage Checkpoint Inhibitors with Chemokine Gradients to Lure and Destroy Pediatric Brain Tumor Cells
Pediatric brain tumors (PBTs) are the leading cause of cancer-related death in children. These malignancies tend to occur in locations of the brain where complete resection and adjuvant therapy could lead to an impaired ... -
Evolutionary adaptations in developmental signaling pathways underlie regenerative scar-free wound repair in African Spiny Mouse (Genus Acomys)
Vertebrate tissue regeneration is a property restricted to a small number of species within the teleost and urodele clades. Contrary to these assumptions, however, all mammalian species examined are also capable of ... -
Fas/FADD-induced pro-inflammatory response in vascular smooth muscle cells
(2002)Apoptosis of smooth muscle cells is a common feature of vascular lesions. Unlike necrosis, apoptosis is classically considered to be "silent," i.e., self-contained and non-inflammatory. I have found that signals initiated ... -
The functions of FE65 proteins and their roles in dementias of the Alzheimer type
(2003)Dementias of the Alzheimer type (DAT) are human neurodegenerative diseases that debilitate millions of elderly people, presenting enormous financial and emotional burdens worldwide. Its pathogenesis involves the beta-amyloid ... -
Genomic Instability at Single Cell Resolution
All organisms maintain the integrity of their genome through highly precise DNA replication and repair. Errors in these mechanisms can lead to genetic instability that results in cellular dysfunction or malignancy. Modern ... -
Growth Signaling in Aging and Disease
(2013-11-14)Nutrient sensing and growth signaling pathways are key regulators of healthspan and longevity. Caloric restriction, the most widely studied and well documented intervention in aging, functions largely through effects on ... -
Human Embryonic Stem Cell-Derived Cardiomyocytes Migrate in Response to Gradients of Fibronectin and Wnt5a: Implications for cardiac repair & congenital heart defects
(2013-11-14)An improved understanding of the factors that regulate the migration of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) would provide new insights into human heart development and suggest novel strategies to ... -
Identification of hypothalamic neural circuits regulating glucose and energy homeostasis
Diabetes and obesity are among the most common and costly health issues facing modern humans. The World Health Organization (WHO) estimates that obesity rates have tripled in the last 50 years: as of 2016, nearly 40% of ...