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Effects of repetitiveness, pairing and linkage on position-effect variegation in Drosophila

Show simple item record Sabl, Joy F en_US 2009-10-05T22:55:49Z 2009-10-05T22:55:49Z 1996 en_US
dc.identifier.other b36972095 en_US
dc.identifier.other 36300610 en_US
dc.identifier.other Thesis 44486 en_US
dc.description Thesis (Ph. D.)--University of Washington, 1996 en_US
dc.description.abstract Position-effect variegation (PEV) is a long distance silencing effect that results in variable inactivation of one or many genes. PEV is caused by the abnormal juxtaposition of heterochromatin and euchromatin following chromosome breakage. By using artificially constructed transgene arrays, I have found that the effects of PEV can be modified by changes in the local repetitiveness of a genetic region. Interestingly, this modification takes place at a distance from the breakpoint, suggesting that the control of variegation does not occur only at the point of propagation. I here propose a new model, emphasizing the role of paired repetitive elements, to account for heterochromatization and for the propagation of PEV. In addition, by using the phenomenon of dominant PEV, I compare the relative contribution of intrachromosomal and allelic pairing to trans-silencing in Drosophila, look at the effects of chromosomal location on dominant PEV, and examine a large array that does not seem to be prone to variegation, in spite of its repetitive nature. Finally, I review the history of chromosomal pairing phenomena and suggest a mechanism that might unify many known pairing and homology-sensing effects in diverse organisms. en_US
dc.format.extent vii, 208 p. en_US
dc.language.iso en_US en_US
dc.rights Copyright is held by the individual authors. en_US
dc.rights.uri en_US
dc.subject.other Theses--Biology en_US
dc.title Effects of repetitiveness, pairing and linkage on position-effect variegation in Drosophila en_US
dc.type Thesis en_US

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