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dc.contributor.authorSabl, Joy Fen_US
dc.date.accessioned2009-10-05T22:55:49Z
dc.date.available2009-10-05T22:55:49Z
dc.date.issued1996en_US
dc.identifier.otherb36972095en_US
dc.identifier.other36300610en_US
dc.identifier.otherThesis 44486en_US
dc.identifier.urihttp://hdl.handle.net/1773/5204
dc.descriptionThesis (Ph. D.)--University of Washington, 1996en_US
dc.description.abstractPosition-effect variegation (PEV) is a long distance silencing effect that results in variable inactivation of one or many genes. PEV is caused by the abnormal juxtaposition of heterochromatin and euchromatin following chromosome breakage. By using artificially constructed transgene arrays, I have found that the effects of PEV can be modified by changes in the local repetitiveness of a genetic region. Interestingly, this modification takes place at a distance from the breakpoint, suggesting that the control of variegation does not occur only at the point of propagation. I here propose a new model, emphasizing the role of paired repetitive elements, to account for heterochromatization and for the propagation of PEV. In addition, by using the phenomenon of dominant PEV, I compare the relative contribution of intrachromosomal and allelic pairing to trans-silencing in Drosophila, look at the effects of chromosomal location on dominant PEV, and examine a large array that does not seem to be prone to variegation, in spite of its repetitive nature. Finally, I review the history of chromosomal pairing phenomena and suggest a mechanism that might unify many known pairing and homology-sensing effects in diverse organisms.en_US
dc.format.extentvii, 208 p.en_US
dc.language.isoen_USen_US
dc.rightsCopyright is held by the individual authors.en_US
dc.rights.urien_US
dc.subject.otherTheses--Biologyen_US
dc.titleEffects of repetitiveness, pairing and linkage on position-effect variegation in Drosophilaen_US
dc.typeThesisen_US


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