Flow injection methods for drug-receptor interaction studies, based on probing cell metabolism

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Flow injection methods for drug-receptor interaction studies, based on probing cell metabolism

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dc.contributor.author Lähdesmäki, Ilkka Johannes en_US
dc.date.accessioned 2009-10-06T21:56:28Z
dc.date.available 2009-10-06T21:56:28Z
dc.date.issued 1999 en_US
dc.identifier.other b43952306 en_US
dc.identifier.other 44070173 en_US
dc.identifier.other Thesis 48912 en_US
dc.identifier.uri http://hdl.handle.net/1773/8590
dc.description Thesis (Ph. D.)--University of Washington, 1999 en_US
dc.description.abstract The thesis describes the development of methods to study responses of living cells to chemical stimulation. To measure the responses, the developed methods utilize effects that the responses have on the rate of the cells' energy metabolism. The metabolic rate is probed either by measuring acid production or oxygen consumption. The technique of flow injection is used throughout the work to achieve swift and reproducible stimulant delivery and removal. Bead injection, a later variant of flow injection, is used to create optimal conditions for the detection of extracellular acidification and oxygen consumption. Detection of pH and [O2] is carried out by the appropriate fluorescent probes and a fluorescence microscope. The methods are validated by carbachol stimulation of Chinese Hamster Ovary M1 (CHO M1) cells. en_US
dc.format.extent x, 114 p. en_US
dc.language.iso en_US en_US
dc.rights.uri en_US
dc.subject.other Theses--Chemistry en_US
dc.title Flow injection methods for drug-receptor interaction studies, based on probing cell metabolism en_US
dc.type Thesis en_US


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