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dc.contributor.authorLähdesmäki, Ilkka Johannesen_US
dc.date.accessioned2009-10-06T21:56:28Z
dc.date.available2009-10-06T21:56:28Z
dc.date.issued1999en_US
dc.identifier.otherb43952306en_US
dc.identifier.other44070173en_US
dc.identifier.otherThesis 48912en_US
dc.identifier.urihttp://hdl.handle.net/1773/8590
dc.descriptionThesis (Ph. D.)--University of Washington, 1999en_US
dc.description.abstractThe thesis describes the development of methods to study responses of living cells to chemical stimulation. To measure the responses, the developed methods utilize effects that the responses have on the rate of the cells' energy metabolism. The metabolic rate is probed either by measuring acid production or oxygen consumption. The technique of flow injection is used throughout the work to achieve swift and reproducible stimulant delivery and removal. Bead injection, a later variant of flow injection, is used to create optimal conditions for the detection of extracellular acidification and oxygen consumption. Detection of pH and [O2] is carried out by the appropriate fluorescent probes and a fluorescence microscope. The methods are validated by carbachol stimulation of Chinese Hamster Ovary M1 (CHO M1) cells.en_US
dc.format.extentx, 114 p.en_US
dc.language.isoen_USen_US
dc.rightsCopyright is held by the individual authors.en_US
dc.rights.urien_US
dc.subject.otherTheses--Chemistryen_US
dc.titleFlow injection methods for drug-receptor interaction studies, based on probing cell metabolismen_US
dc.typeThesisen_US


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