Carothers, James MXi, Chenggang2019-08-142019-08-142019Xi_washington_0250O_20072.pdfhttp://hdl.handle.net/1773/44094Thesis (Master's)--University of Washington, 2019Biological parts can be considered as molecular building blocks to assemble complex synthetic genetic networks utilizing synthetic biology. In this work, we constructed three types of multi-Ribozyme-gRNA-Aptazyme (RGA) networks for biosensing and gene regulation by linking RGA, Ribozyme-gRNA-Ribozyme (RGR) and CRISPRi systems together. We screened out two different aptazymes that fit this network and analyzed their one-layer and two-layer single-input RGAs behaviors and functions in two-inputs systems with different architectures. Overall, we built two IMPLY gates, two NIMPLY gates, one AND gate, one partial NAND gate, one conditional non-Boolean gate. The RGA shows as much as a 7.38-fold increase in ligand-dependent system-output. The interaction between RGAs that are inserted in circuits in parallel has also been reported, which contributions to the predictability of more complicated RGA networks.application/pdfen-USnoneAptazymeCRISPRigenetic circuitgRNAChemical engineeringBioengineeringChemical engineeringThesis