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oai:digital.lib.washington.edu:1773/261762026-02-15T21:17:20Zcom_1773_4888col_1773_4943

Queitsch, Christine Lachowiec, Jennifer Anna 2014-10-13T16:58:58Z 2015-12-14T17:55:52Z 2014-10-13 2014 Lachowiec_washington_0250E_13725.pdf http://hdl.handle.net/1773/26176 Thesis (Ph.D.)--University of Washington, 2014 Phenotypes are buffered from both genetic perturbations and developmental noise; however, the mechanisms by which this buffering occurs and its evolutionary relevance are poorly understood. In this dissertation, a combination of genetic and computational approaches were undertaken to not only identify genetic loci that are buffered from phenotypes but also map genes that provide phenotypic buffering. For example, I found that substrates of Heat shock protein 90 (Hsp90), the best-understood source of buffering, tend to accumulate genetic changes in a manner that affects evolution. Hsp90 is also a proven buffer of developmental noise, so the mechanism by which this ability arises was explored. To expand the number of known developmental noise buffers, innovative methods for genome-wide association were used to map novel regulators. The ability to identify loci that are buffered and provide buffering indicates that the distribution of phenotypes across a population arises through complex interactions between genetic loci, including genes that act as buffers. application/pdf en_US Copyright is held by the individual authors. buffering; developmental noise; evolution; genetic variation; robustness Evolution & development Molecular biology Genetics molecular and cellular biology Tracking buffers of mutation and noise to the genome Thesis Restrict to UW for 1 year -- then make Open Access open.access