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oai:digital.lib.washington.edu:1773/330682026-02-16T02:32:41Zcom_1773_4888col_1773_4903

Jiang, Shaoyi Nowinski, Ann Kate 2015-05-11T19:56:04Z 2015-05-11 2015 Nowinski_washington_0250E_14173.pdf http://hdl.handle.net/1773/33068 Thesis (Ph.D.)--University of Washington, 2015 This dissertation is a compilation of efforts aimed at developing mixed charge and zwitterionic materials to prevent biofouling in biomedical applications. Peptides are considered for use as natural low fouling materials due to their biocompatibility, diverse chemical properties, well-defined sequence, and biodegradability. Low fouling peptide sequences were rationally designed from known zwitterionic principles and biomimetically designed from the examination of human protein surfaces found in nature. The peptide sequences glutamic acid/lysine (EK) and asparagine (N) were identified as displaying resistance to nonspecific protein adsorption. The low fouling sequence EK was further applied to several biomedical applications including multifunctional self-assembled monolayers on a gold surface, stealth coatings on gold nanoparticles, and biodegradable EK-poly(lactic-co-glycolic) acid (EK-PLGA) drug delivery nanocarriers. Importantly, the EK sequence can be extended with functional peptide sequences (such as RGD for specific cell targeting) without the need for complex chemical conjugation. In addition to rational and biomimetic peptide design, a combinatorial screening method was explored to facilitate the testing of thousands of peptide sequences for low protein adsorption. Finally, zwitterionic polymers were explored to form particles for mucus-penetrating applications. application/pdf en_US Copyright is held by the individual authors. Combinatorial Design; Low fouling; Mucus Penetration; Nanoparticle; Peptides; Zwitterionic polymers Chemical engineering Biomedical engineering Materials Science Chemical engineering Developing Low Fouling Peptides and Zwitterionic Materials for Biomedical Applications Thesis Delay release for 1 year -- then make Open Access 2016-05-10T19:56:04Z open.access