Optimization of Multiparametric Flow Cytometry Assays: A Comprehensive Assessment of Immunophenotypic Characteristics of Monocytic and Blastic Plasmacytoid Dendritic Cell Neoplasms

Abstract

This study aimed to design and optimize multiparametric flow cytometry (MFC) assays forin-depth characterization of monocytic cells and plasmacytoid dendritic cells (pDCs) in the context of hematological neoplasms, such as monocytic leukemia and blastic plasmacytoid dendritic cell neoplasm (BPDCN). Building on previous research, we integrated novel markers, including LILRB1, LILRB4, and CD300e for the monocytic assay panel and CD303 and CD304 for the pDC assay panel, to provide a comprehensive characterization of antigenic expression associated with maturation and differentiation of these populations. We established unique expression patterns of novel markers and highlighted distinctive immunophenotypic features of monocytic cells and pDCs in their normal and neoplastic state, demonstrating robust performance and reliability of the optimized assays.