The Analysis of Photochemical Nitrile Imine Cross-Linking for Ribonucleosides in Gas-Phase Ions

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dc.contributor.advisorTurecek, Frantisek
dc.contributor.authorWei, Chenyang
dc.date.accessioned2025-08-01T22:18:30Z
dc.date.available2025-08-01T22:18:30Z
dc.date.issued2025-08-01
dc.date.submitted2025
dc.descriptionThesis (Master's)--University of Washington, 2025
dc.description.abstractThis research presents the exploration of nitrile imine cross-linking related to ribonucleoside ions in the gas phase. Riboguanosine was tagged with 4-(2-phenyl-2H-tetrazol-5-yl)benzoic acid at the 5’O-ribose position. The tagged nucleosides were analyzed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry including ultraviolet photodissociation tandem mass spectrometry (UVPD-MS) and collision-induced dissociation tandem mass spectrometry (CID-MS). Crosslinking was found to occur in tetrazole-guanosine ions upon N2 loss under CID-MS2 and UVPD-MS2. The spectra from CID-MS3 and UVPD-CID-MS3 also suggested that the cross-linking involved interactions between the guanine carbonyl group and the nitrile imine.
dc.embargo.termsOpen Access
dc.format.mimetypeapplication/pdf
dc.identifier.otherWei_washington_0250O_28040.pdf
dc.identifier.urihttps://hdl.handle.net/1773/53474
dc.language.isoen_US
dc.rightsnone
dc.subjectChemistry
dc.subject.otherChemistry
dc.titleThe Analysis of Photochemical Nitrile Imine Cross-Linking for Ribonucleosides in Gas-Phase Ions
dc.typeThesis

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