Nonlinear optical microscopy for tissue imaging and analysis in anatomic pathology

Abstract

Stimulated Raman Scattering (SRS) microscopy provides chemical information for imaged tissue at submicron resolution. In the experiments described, I combine SRS with other non-linear microscopies, including second harmonic generation (SHG) microscopy and two-photon fluorescence (TPF) microscopy, to develop a methodology for tissue imaging and analysis with anatomic pathology diagnostic challenges in mind. Employing an interdisciplinary approach in collaboration with experts in the field I investigate the diagnostic potential of a multimodal approach to complex tissues such as brain, breast, and bone, illuminating the immense potential non-linear optical microscopy has to offer to pathologists and clinicians as they strive to improve patient care.