Cell-specific transcriptional analysis in complex tissues

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dc.contributor.advisorAltemeier, William A
dc.contributor.authorMcKinney, Bonnie Lewis
dc.date.accessioned2017-02-14T22:41:52Z
dc.date.issued2017-02-14
dc.date.submitted2016-12
dc.descriptionThesis (Master's)--University of Washington, 2016-12
dc.description.abstractCell-type specific transcriptional analysis can yield important information about the role of a certain cell type in the context of its tissue microenvironment. Previous methods for isolating cell type-specific transcriptional data have included enzymatic or mechanical tissue dispersion followed by magnetic cell sorting (MACS) or fluorescence-activated cell sorting (FACS) and laser capture microdissection. However, these methods have important limitations. Transcriptome analysis by translating ribosome affinity purification (TRAP) allows the researcher to isolate translating mRNA from a genetically defined cell type. Discussed in this review are current methods for translating ribosome affinity purification, caveats of this method, and considerations for successfully completing these studies.
dc.embargo.lift2018-02-14T22:41:52Z
dc.embargo.termsRestrict to UW for 1 year -- then make Open Access
dc.format.mimetypeapplication/pdf
dc.identifier.otherMcKinney_washington_0250O_16615.pdf
dc.identifier.urihttp://hdl.handle.net/1773/38194
dc.language.isoen_US
dc.rightsnone
dc.subjectLung stromal cells
dc.subjectRibotag
dc.subjectTranslating ribosome affinity purification
dc.subject.otherPathology
dc.subject.otherMedicine
dc.subject.otherpathology
dc.titleCell-specific transcriptional analysis in complex tissues
dc.typeThesis

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